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Yes, Home Depot and Lowes both sell 1/4" thick acrylic. Not very large sizes though, I think the biggest is 24" x 48", but I could be wrong.
What are you using the acrylic for? Do you need sheets, rods, cylinders? Give a bit more details and I can suggest some online places that carry what you need. It's real tough to find a local place (other than the "big 2" above.)